This method has allowed to create a new class of matrices on the basis of biocompatible cross-linked hydrogels which imitate extracellular matrix. It should be noted that the tissues of blood vessels with high cell density could be created without the use of any bioreactor. Those investigations has got further advance in the use of the laser for acellular framework perforation. That allows to obtain micropores of optimal size and density without changing mechanical properties of structures. Using acellular small-intestinal submucosa as a framework, the scientists have obtained micropores which are 50 µm in diameter, when distances between the pores are 714 µm. To achieve recellularization of that matrix, rotary filling of living cells suspended in hyaluronic hydrogel is used. The next stage in bioengineering of vascular tissue will be connected with the use of nanotechnological methods. The method allows to obtain bioengineered tough tubular constructions without using any reactor.
Place of employment — MUSC Bioprinting Center Medical University of South Carolina, USA
Contacta — Bioprinting Research Center, Cardiovascular Developmental Biology Center, Department of Cell Biology and Anatomy, Medical University of South Carolina, 173 Ashley Ave., Charleston, SC 29425, USA.(843) 792 7630mironovv@musc.eduhttp://organprint.missouri.edu/www/mironov.php.
Publications — Organ printing: tissue spheroids as building blocks. Mironov v. , Visconti R.P., Kasyanov v. , Forgacs G., Drake C.J., Markwald R. R. Biomaterials. 2009 Apr;30(12):2164–74 Bioreactor-free tissue engineering: directed tissue assembly by centrifugal casting. Mironov v. , Kasyanov v. , Markwald R.R., Prestwich G. D. Expert Opin. Biol. Ther. 2008 Feb;8(2):143–52 Rapid biofabrication of tubular tissue constructs by centrifugal casting in a decellularized nat-ural scaffold with laser-machined micropores. Kasyanov v. A., Hodde J., Hiles M.C., Eisenberg C., Eisenberg L., De Castro L.E., Ozolanta I., Murovska M., Draughn R.A., Prestwich G.D., Markwald R.R, Mironov v. J Mater. Sci. Mater. Med. 2009 Jan;20(1):329–37 Review: bioprinting: a beginning. Mironov V, Reis N, Derby B. Tissue Eng. 2006 Apr;12(4):631–4.
